About the only difference is the addition of more contemporary references in the punchlines (David Beckham, The Situation, "You Got Served," AOL, Viagra and, most memorably, ShamWow), not to mention subtle jokes involving gay marriage and abstinence rings.
Still, what's funny then is funny now, because Reubens commits so admirably to the character. Although the running time is feature-length, the show is too loose in plot to deliver quite the same jolt of satisfaction as a movie (especially since director Marty Callner doesn't always have the timing to match the action), but until that day comes, this'll do.
As Pee-wee repeats many a time, "No worries, same difference, it's all good." —Rod Lott